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This expert publication provides a comprehensive overview of oral biofilms from both clinical and translational research perspectives. It details the formation of organized polymicrobial communities on natural teeth, restorative materials, and dental implants. The content covers critical topics including periodontal biofilms, caries-related infections, and the efficacy of various anti-biofilm therapies. This resource serves as an essential guide for dental students, practitioners, and scientists focusing on oral healthcare advancements.